出品公司: | Promega |
---|---|
载体名称: | pNL1.3[secNluc] |
质粒类型: | 荧光素酶表达载体 |
高拷贝/低拷贝: | -- |
克隆方法: | --- |
启动子: | -- |
载体大小: | 3194 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | 无启动子载体,用于通过分泌的NanoLuc®荧光素酶检测启动子和增强子序列的活性。 |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pNL1.3[secNluc]载体质粒基本信息
pNL1.3[secNluc]质粒图谱载体图谱和pNL1.3[secNluc]载体序列质粒序列多克隆位点信息
pNL1.3[secNluc]质粒载体简介
pNL1.3[secNluc]质粒序列载体序列
LOCUS pNL1.3[secNluc] 3194 bp ds-DNA circular SYN 26-JUL-2013 DEFINITION Promoterless vector for measuring the activity of promoter and enhancer sequences with a secreted NanoLuc(R) luciferase assay. ACCESSION JQ437372 VERSION . KEYWORDS pNL1.3[secNluc] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3194) AUTHORS Promega TITLE Direct Submission JOURNAL Exported Thursday, February 20, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3194 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" misc_feature 1..70 /note="MCS" /note="multiple cloning site" CDS 100..186 /codon_start=1 /product="secretion signal from interleukin 6" /note="IL-6 signal sequence" /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP" CDS 187..699 /codon_start=1 /product="NanoLuc(R) luciferase" /note="Nluc" /note="human codon-optimized" /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR LCERILA" polyA_signal 748..869 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1288..1876) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2076..2936) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 3041..3089 /note="synthetic polyadenylation signal" misc_feature 3103..3194 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgaactcctt ctccacaagc 121 gccttcggtc cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct 181 gccccagtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac 241 ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc 301 gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat 361 gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt 421 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg 481 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc 541 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt 601 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga 661 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag gccgcgactc tagagtcggg 721 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 781 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 901 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 961 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc 1021 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa 1081 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc 1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 1261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 2041 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca 2101 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag 2161 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag 2221 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga 2281 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct 2341 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc 2401 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc 2461 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc 2521 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat 2581 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag 2641 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac 2701 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg 2761 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca 2821 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc 2881 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc 2941 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa 3001 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc 3061 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca 3121 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc 3181 cagaacattt ctct //